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反轉(zhuǎn)錄酶

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產(chǎn)品名稱: 反轉(zhuǎn)錄酶
產(chǎn)品型號: invitrogen 18080-044
產(chǎn)品展商: Invitrogen
產(chǎn)品文檔: 無相關(guān)文檔

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SuperScript III Reverse Transcriptase 反轉(zhuǎn)錄酶III 逆轉(zhuǎn)錄酶 美國invitrogen公司原裝進口


反轉(zhuǎn)錄酶  的詳細介紹

SuperScript III Reverse Transcriptase
反轉(zhuǎn)錄酶III     逆轉(zhuǎn)錄酶

Description

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability. The structural modifications provide:
  • Half life of 220 minutes at 50°C for the highest cDNA yields (Figure 1)
  • Reduced RNase H activity for more full-length cDNA
  • Full activity at 50°C for increased specificity with GSP
  • Ability to increase RT units without inhibiting subsequent PCR
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