Periodate Treatment of Fixed Cell Plates
1. Reagents
Sodium Borohydride (NaBH4) - 1 mg/ml
Carbonate:Bicarbonate Buffer, pH 9.6
Sodium Periodate (NaIO4) - 1 mM, 2.14 mg/10 ml Acetate Buffer
10mM Acetate Buffer, pH 4.5
0.82 g sodium acetate in 90 ml diH2O.
Adjust to pH 4.5 with glacial acetic acid. Q.S. to 100 mlwith diH2O.
2. Procedure
To one-half the plate add 100 ml/well periodate. To theother half add 100 ml/well acetate buffer.
Incubate 2 hr at 37oC.
Wash 2X with 0.1M bicarbonate buffer.
Add 100 ml/well sodium borohydride.
Incubate 30 min, RT
Wash plate 2X with PBS
Block plate as required by assay protocol.
3. Notes
In fixed cell ELISAs. do not use carbonate wash beforesubstrate.
