產(chǎn)品名稱(chēng)：Lyme Borrelia canine IgG ELISA 產(chǎn) 地：Demeditec 產(chǎn)品貨號(hào)：DE0320V 產(chǎn)品規(guī)格：96 Tests 產(chǎn)品說(shuō)明： Special remarks: For research use only! INTRODUCTION Lyme disease (Borreliosis) is a tick-borne, bacterial disease of domestic animals and humans. It is caused by spirochetes of the Borrelia burgdorferi sensu lato group. In Europe, the most frequently isolated pathogenic genospecies of humans and dogs are Borrelia afzelii, Borrelia garinii and Borrelia burgdorferi sensu stricto. Borrelia are helically wound, flexible, highly motile bacteria. By rotation of their axial filaments (periplasmatic flagella) they are able to move efficiently in corkscrew fashion through viscous media (serum). Thereby they can disseminate throughout the body within days to weeks of infection. The pathogens are transmitted by various tick species of the genus Ixodes. In Europe, Ixodes ricinus is the most important vector. However, infestation rates with Borrelia vary depending on the region. In endemic areas of Germany, approximately 3-7 % of the larvae and 10-34 % of nymphs and ***** ticks are infected by Borrelia burgdorferi sensu lato. Natural reservoirs are wild animals, including rodents as well as many other small mammals and birds. The ticks take their meals (blood) from these hosts. Since dogs frequent the areas ticks live, they are more affected than humans. Typical tick habitats are the edge of the woods, bushes, undergrowth and tall grass; but infected ticks can also be found in public parks. Symptoms of Lyme disease in dog comprise fever, apathy, loss of appetite and anorexia as well as recurrent and shifting lameness and polyarthritis. The characteristic rash or the circular area of redness around the bite (erythema chronicum migrans) which is seen in man may be absent or is overlooked due to hair coat or dark pigmentation. PRINCIPLE OF THE ASSAY The qualitative immunoenzymatic determination of IgG-class antibodies against Borrelia is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are pre-coated with Lyme Borrelia antigens incl. VlsE to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-dog IgG conjugate is added. This conjugate binds to the captured Borrelia-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine- (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Borrelia-specific IgG antibodies in the specimen. Acidic solution is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader. 上海玉博生物技術(shù)有限公司在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國(guó)內(nèi)**地位，公司提供的產(chǎn)品涵蓋了二十多個(gè)國(guó)家近五十萬(wàn)種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長(zhǎng)和更新，公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對(duì)生物化學(xué)，分子生物學(xué)，細(xì)胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認(rèn)知，以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。
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