產(chǎn)品名稱：Melatonin in saliva ELISA 產(chǎn) 地：Demeditec 產(chǎn)品貨號(hào)：DEKDSM96 產(chǎn)品規(guī)格：96 Tests 產(chǎn)品說明： Special remarks: The Direct Saliva Melatonin ELISA is a competitive immunoassay using a capture antibody (Ab) technique. The polyclonal Kennaway G280 anti-melatonin antibody has been coated onto the microtiter plate, provided in the kit. After the first 16-20 hours overnight incubation, melatonin present in the pre-treated saliva and controls as well as in the calibrators, compete with biotinylated melatonin during a second 3 hours incubation for the binding sites of this highly specific antibody. After washing, the enzyme label, streptavidin conjugated to horseradish peroxidase (HRP) is added, which binds during a third 60 minutes incubation step to the melatonin-biotin antibody complexes captured on the coated wells. Unbound enzyme label is then removed by a second washing step and TMB substrate (tetramethylbenzidine) is added to the wells. In a fourth 30 minutes incubation step, a chromophore is formed in inverse proportion to the amount of melatonin present in the sample. The color turns from blue to yellow after the addition of an acidic stop solution and can be measured at 450 nm. 上海玉博生物技術(shù)有限公司在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國(guó)內(nèi)**地位，公司提供的產(chǎn)品涵蓋了二十多個(gè)國(guó)家近五十萬種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長(zhǎng)和更新，公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對(duì)生物化學(xué)，分子生物學(xué)，細(xì)胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認(rèn)知，以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。
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