產(chǎn)品名稱：Osteocalcin mouse 產(chǎn) 地：Demeditec 產(chǎn)品貨號：DEBT470 產(chǎn)品規(guī)格：96 Tests 產(chǎn)品說明： Special remarks: Osteocalcin, the vitamin K-dependent protein of bone, is a specific product of the osteoblast. It is distinguished by its small size (5800 daltons) and the presence of gamma-carboxy-glutamic acid (Gla). In the presence of ionic calcium, the Gla residues allow a specific conformational change in the protein, which in turn promotes osteocalcin binding to bone mineral and subsequent accumulation in bone matrix. While osteocalcin is primarily deposited into the extracellular matrix of bone, a small amount can be detected in the blood. Circulating osteocalcin is thought to reflect that portion of newly synthesized protein that does not bind to bone but is released directly into the circulation. This sandwich ELISA Kit is specific for mouse osteocalcin only. Both carboxylated and decarboxylated mouse osteocalcin are recognized. Mouse osteocalcin can be measured directly from serum, heparinized plasma, bone extracts and cell culture supernates. A polyclonal antibody directed against the N-terminus is bound to the polystyrene wells. An overnight incubation is done with sample and biotinylated antibody specific for the C-terminus. Detection is achieved after washing and a short incubation with Peroxidase-Streptavidin Conjugate.Standards of highly purified Mouse Osteocalcin are used to generate a standard curve. The design of the animal experiment is most important. Many variables effect serum osteocalcin levels: age, growth rate, hormonal status, vitamin D intake, stress, circadian rhythm, etc. It is desirable to take blood samples under the same conditions: time of day, without stress, etc. and process the serum (or plasma) the same way for each sampling. We have found a range of 40-150 ng/ml in mouse serum that we evaluated. Young mice (<60 days) can have levels of 500ng/ml or more. Concentrations of osteocalcin in conditioned media range from <1ng/ml to 400ng/ml depending on the cell type and culture conditions. Full dilution series should be done to establish linearity of dilution. Medium and serum interference should be assessed by appropriate blanks and internal standards of medium with added mouse osteocalcin. Fetal Bovine Serum shows little interference. Other species of sera should be tested. Culture media can generally be assayed directly. This test also allows the measurement of osteocalcin in EDTA extarcts of bone powder after appropriate dilution. Typical levels are 1 - 2 ng per microgram of dry bone powder. 上海玉博生物技術(shù)有限公司在為生命科學領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國內(nèi)**地位，公司提供的產(chǎn)品涵蓋了二十多個國家近五十萬種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長和更新，公司提供的產(chǎn)品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質(zhì)組學研究的認知，以及分子診斷和臨床醫(yī)學領(lǐng)域的應用。
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