LH rat ELISA
產(chǎn)品名稱：LH rat ELISA
產(chǎn) 地：Demeditec
產(chǎn)品貨號(hào)：DEAKRLH010
產(chǎn)品規(guī)格：96 Tests
產(chǎn)品說明：
Special remarks: For research use only!
Introduction
LH is produced and stored in basophilic cells called gonadotrophs in the anterior pituitary gland. LH is found in all vertebrates from fishes to mammals. Some reports indicated the presence of LH-like substance in the testis. LH has a heterodimer structure consisted of α-subunit and β-subunit with a total molecular weight of approximately 29,000. α-Subunit is a glycoprotein which is common to other pituitary hormones follicle-stimulating hormone (FSH) and thyroid-stimulating hormone (TSH), while β-subunit is specific to LH, and is also a glycoprotein. The receptor of LH is a transmembrane receptor of G-protein coupled type, which relates to PKA system and penetrates the cell membrane 7 times. In females, LH acts on maturated granulosa cells in ovarian follicle, where it causes, in cooperation with FSH, follicular maturation, estrogen production, and ovulation, and after ovulation, acts on corpora lutea and promotes progesterone production and secretion. In males, LH acts on the interstitial cells (Leidig cells) in the testis, causing production and secretion of androgens, and secondarily promotes spermatogenesis via androgen. Deficiency of LH leads to the lower secretion of sex steroids, atrophy of interstitial cells, and failure of ovulation and luteinization, while excessive LH causes hyperplasia of testicular interstitial cells followed by atrophy, increased secretion of estrogen or androgen, super-ovulation, and accelerated sexual maturation. GnRH (LHRH) secreted from hypothalamus act directly on gonadotrophs and stimulate LH secretion. Physiologically, lowered sex steroids blood levels, sexual cycle especially positive feedback of estrogen at preovulatory period causing LH surge via LHRH and leading to ovulation, postmenopausal period, aging in men. LH secretion was minimized by increased blood levels of sex steroids, opioid peptides especially endorphin, childhood, and pregnancy.
Assay principle
In Demeditec LH rat ELISA Kit, standards or samples are incubated in monoclonal anti-LH α antibody-coated wells to capture LH. After 2 hours’ incubation and washing, biotin-labeled anti-LH β antibody is added and incubated further for 1 hours to bind with captured LH. After washing, HRP (horse radish peroxidase)-labeled avidin is added, and incubated for 30 minutes. After washing, HRP-complex remaining in wells is reacted with a chromogenic substrate (TMB) for 20 minutes, and reaction is stopped by addition of acidic solution, and absorbance of yellow product is measured spectrophotometrically at 450 nm. The absorbance is proportional to LH concentration. The standard curve is prepared by plotting absorbance against standard LH concentrations. LH concentrations in unknown samples are determined using this standard curve.
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