產(chǎn)品名稱：ENAscreen ELISA 產(chǎn) 地：Demeditec 產(chǎn)品貨號：DE7420 產(chǎn)品規(guī)格：96 Tests 產(chǎn)品說明： Special remarks: ENAscreen is an indirect solid phase enzyme immunoassay (ELISA) for the qualitative screening of IgG class autoantibodies against extractable nuclear antigens (ENA) in human serum or plasma. The assay is intended for in vitro diagnostic use only as an aid in the diagnosis of rheumatic diseases such as systemic lupus erythematosus (SLE), Sjögren´s syndrome, scleroderma and mixed connective tissue disease. Rheumatoid autoimmune diseases are often associated with the occurence of autoantibodies against several nuclear or cytoplasmatic antigens. These so-called anti nuclear antigens (ANA) can be devided into three groups: 1.true anti nuclear antigens (ANA):directed against dsDNA, ssDNA, histones, nucleolic RNA and DNP 2.extractable nuclears antigens:Sm (Smith), n-RNP, Scl 70, Jo-1 and PM-1 3.cytoplasmatic antigens:SS-A (Ro) and SS-B (La) In patients with Sjögren-Syndrome antibodies against the two cytoplasmatic antigens often occur in combination. Due to their strong association of SS-A and SS-B antibodies to the HLA-DR3 and DR2 phenotypes a genetic predisposion is suspected. The anti SS-A protein passes the placenta and may cause the development of SLE in neonates. Immunoreactive proteines may occur in various combinations and also bound to 'host proteins' of viral origin. They induce synthesis of polyclonal autoantibodies, of the IgG, IgM and IgA class of immunoglobulins. Especially for mixed connective tissue diseases a relation to viral infections by EBV (Eppstein-Barr-Virus) is indicated. Each class of immunoglobulins causes specific immune fluorescent pattern. Basically immunofluorescence titers correlate with the quantitation of IgG antibodies but the concentrations may considerably vary within each titer. Quantitation of IgG class antibodies extensively correlate with the diseases' activity. This makes it superior to immuno-fluorescence using HEp 2 cells, which may give variable results depending on their degree of activity. Also IF with Crithidia lucillae sometimes gives discrepant results. Today the best investigated immunoreactive antigens are double-stranded DNA (dsDNA), single stranded DNA (ssDNA), Sm (Smith), sn-RNP (small nuclear ribonucleoprotein particles), the complex RNP/Sm which is stabilized by ribonuleic acid as well as SS-A (Ro) and SS-B (La). The antigen Scl 70, a 70 kD molecular weight protein is associated with scleroderma. In rheumatoid autoimmune diseases various profiles of autoantibodies to these antigens can be detected. In a high incidence they are related to active and inactive systemic Lupus erythrematodes, mixed connective tissue diseases (Sharp Syndrome), rheumatoid arthritis, Sjögren-Syndrome, Sclerodermia, photosensitve dermatitis and drug-induced lupus. In Lupus patients typically anti-dsDNA antibodies can be detected. Patients without these antibodies very often show anti-ssDNA antibodies and anti-SS-A and anti-SS-B are present. A strong correlation between antibody concentration and severety of the disease has been observed with higher antibody concentrations in active phases of the disease. Thus quantitation is more informative compared to simple titering by immunofluorescence. Most of these parameters are not specific for just one disease but they occur in various combinations. The pattern of different antibody combinations and their concentration together with the whole clinical picture of the patient are helpful diagnostic tools in the assessment of rheumatoid autoimmune diseases. 上海玉博生物技術(shù)有限公司在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國內(nèi)**地位，公司提供的產(chǎn)品涵蓋了二十多個(gè)國家近五十萬種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長和更新，公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對生物化學(xué)，分子生物學(xué)，細(xì)胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認(rèn)知，以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。
滬公網(wǎng)安備 31011002002623號
