產(chǎn)品名稱：Hepcidin Prohormone ELISA
產(chǎn)    地：Demeditec 
產(chǎn)品貨號(hào)：DE4644
產(chǎn)品規(guī)格：96 Tests
產(chǎn)品說(shuō)明：
Special remarks:
1 Introduction
The DEMEDITEC Hepcidin Prohormone Enzyme Immunoassay Kit provides materials for the quantitative determination of Hepcidin Prohormone in human serum and urine.
This assay is intended for research use only.
Hepcidin is a small cystein-rich peptide produced in the liver [1]. This molecule regulates the absorption of iron in the body similarly to the correlation of insulin and sugar.
Hepcidin was initially isolated as a 25 amino acid (aa) peptide in human plasma and urine exibiting antimicrobial activity [2,3].
Human Hepcidin is produced from a 84 aa precursor including a putative (24 aa) signal peptide.
Application of the present ELISA allows the detection and determination of Hepcidin Prohormone (Pro?Hepcidin) in serum of patients suffering from several disorders of iron metabolism.
2 Principle of the test
The DEMEDITEC Hepcidin Prohormone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding.
The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site on the Hepcidin Prohormone molecule (28-47 aa). Endogenous Hepcidin Prohormone of a patient sample competes with a Hepcidin Prohormone-biotin conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off.
The amount of bound biotin conjugate is reverse proportional to the concentration of Hepcidin Prohormone in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Hepcidin Prohormone in the patient sample.
上海玉博生物技術(shù)有限公司在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國(guó)內(nèi)**地位，公司提供的產(chǎn)品涵蓋了二十多個(gè)國(guó)家近五十萬(wàn)種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長(zhǎng)和更新，公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對(duì)生物化學(xué)，分子生物學(xué)，細(xì)胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認(rèn)知，以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。

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