產(chǎn)品名稱：Growth Hormone (rat/mouse) ELISA 產(chǎn) 地：Demeditec 產(chǎn)品貨號(hào)：DE4614 產(chǎn)品規(guī)格：96 Tests 產(chǎn)品說(shuō)明： Special remarks: This kit is for research purpose only. INTENDED USE This Rat/Mouse Growth Hormone ELISA kit is used for the non-radioactive quantification of Growth Hormone in rat or mouse serum, plasma, tissue extracts or cell culture media samples. One kit is sufficient to measure 39 unknown samples in duplicate. PRINCIPLES OF PROCEDURE This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone polyclonal antibodies, 2) wash away of unbound materials from samples, 3) binding of a second biotinylated anti-Growth Hormone polyclonal antibody to the captured molecules, 4) wash away of unbound materials from samples, 5) conjugation of horseradish peroxidase to the immobilized biotinylated antibodies, 6) wash away of free enzyme conjugates, and 7) quantification of immobilized antibody-enzyme conjugates by monitoring horseradish peroxidase activities in the presence of the substrate 3,3´,5,5´-tetramethylbenzidine. The enzyme activity is measured spectrophotometrically by the increased absorbency at 450 nm, corrected from the absorbency at 590 nm, after acidification of formed products. Since the increase in absorbency is directly proportional to the amount of captured rat or mouse Growth Hormone in the unknown sample, the latter can be derived by interpolation from a reference curve generated in the same assay with reference standards of known concentrations of Rat Growth Hormone. 上海玉博生物技術(shù)有限公司在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國(guó)內(nèi)**地位，公司提供的產(chǎn)品涵蓋了二十多個(gè)國(guó)家近五十萬(wàn)種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長(zhǎng)和更新，公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對(duì)生物化學(xué)，分子生物學(xué)，細(xì)胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認(rèn)知，以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。
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