產(chǎn)品名稱：C-Peptide IRMA 產(chǎn) 地：Demeditec 產(chǎn)品貨號：DE35100 產(chǎn)品規(guī)格：100 Tests 產(chǎn)品說明： Special remarks: The C-PEPTIDE [125I] IRMA system provides a direct quantitative determination of C-peptide in human serum and urine. C-peptide can be assayed in the range of 0?30ng/ml (in human serum) and 0-300 ng/ml (in urine) using 50 µl serum samples. Each kit contains materials sufficient for 100 assay tubes permitting the construction of one standard curve and the assay of 42 unknowns in duplicate. Introduction C-peptide (connective peptide) is a polypeptide with a molecular weight of 3600,containing 31 amino acid. It derives from proinsulin produced in pancreatic beta cells. Proinsulin is converted to insulin and C-peptide, which are then secreted into the portal blood in an equimolar quantity. Contrary to insulin, C-peptide is not extracted by the liver, but enters the systemic circulation. In the diagnosis of diabetes, C-peptide is a more reliable indicator of insulin secretion than is the insulin itself. Furthermore, the concentration of C-peptide is not affected by interference from insulin antibodies often present in patients receiving insulin therapy. The assessment of residual endogenous insulin secretion by the measurement of C-peptide is an indispensable tool for the diagnosis and treatment of diabetes mellitus. Principle of method The technology uses two high affinity monoclonal antibodies in an immuno- radiometric assay (IRMA) system. The 125I labelled signal-antibody binds to an epitope of the C-peptid molecule, which is different from that recognised by the unlabelled capture-antibody. The two antibodies react simultaneously with the antigen present in standards or samples which leads to the formation of a capture antibody - antigen - signal antibody complex, also referred to as a “sandwich”. During a 3-hour incubation period with continuous agitation immuno-complex is immobilised on the reactive surface of test tubes. Reaction mixture is then discarded, test tubes are washed exhaustively, and the radioactivity is measured in a gamma counter. The concentration of antigen is directly proportional to the radioactivity measured in test tubes. By constructing a calibration curve plotting binding values against a series of standards containing known amount of C-peptid, the unknown concentration of C-peptid in patient samples can determined. 上海玉博生物技術(shù)有限公司在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國內(nèi)**地位，公司提供的產(chǎn)品涵蓋了二十多個國家近五十萬種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長和更新，公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對生物化學(xué)，分子生物學(xué)，細胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認知，以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。
滬公網(wǎng)安備 31011002002623號
