產(chǎn)品名稱:Vasopressin ELISA 產(chǎn) 地:Demeditec 產(chǎn)品貨號(hào):DE3122 產(chǎn)品規(guī)格:96 Tests 產(chǎn)品說明: Special remarks: 1 DESCRIPTION The DEMEDITEC Vasopressin ELISA kit is a competitive immunoassay for the quantitative determination of Vasopressin in samples. The kit uses a polyclonal antibody to Vasopressin to bind, in a competitive manner, the Vasopressin in the sample or an alkaline phosphatase molecule which has Vasopressin covalently attached to it. After a simultaneous incubation at 4ºC the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of Vasopressin in either standards or samples. The measured optical density is used to calculate the concentration of Vasopressin. For further explanation of the principles and practice of immunoassays please see the excellent books by Chard or Tijssen. 2 INTRODUCTION Arginine Vasopressin (AVP) is a 9 amino acid peptide with a 6-member disulfide ring. It is structurally related to oxytocin differing in 2 amino acids. It is synthesized in the hypothalamus supraoptic and paraventricular nuclei. It is stored in the posterior pituitary for release. AVP has powerful antidiuretic action and is also known as antidiuretic hormone (ADH). It acts upon the collecting tubule of the kidney increasing permeability to water and urea. It also has neurotransmitter and peripheral humoral functions. AVP has been shown to be released upon both osmotic and non-osmotic stimuli, and its release into peripheral blood causes effects upon a number of factors, including emotional stress, posture, blood volume, and temperature. Alcohol appears to inhibit AVP secretion. Serum AVP measurement is used clinically for studies involving diabetes insipidus, syndrome of inappropriate ADH secretion (SIADH), ectopic AVP production and pyschogenic water intoxication. 上海玉博生物技術(shù)有限公司在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國內(nèi)**地位,公司提供的產(chǎn)品涵蓋了二十多個(gè)國家近五十萬種產(chǎn)品,而且產(chǎn)品的數(shù)量與信息在不斷的增長和更新,公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對(duì)生物化學(xué),分子生物學(xué),細(xì)胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認(rèn)知,以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。
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