產(chǎn)品名稱：Oxytocin ELISA 產(chǎn) 地：Demeditec 產(chǎn)品貨號：DE3117 產(chǎn)品規(guī)格：96 Tests 產(chǎn)品說明： Special remarks: FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. 1 Description The Oxytocin ELISA kit is a competitive immunoassay for the quantitative determination of Oxytocin in samples. The kit uses a polyclonal antibody to Oxytocin to bind, in a competitive manner, the Oxytocin in the standard or sample or an alkaline phosphatase molecule which has Oxytocin covalently attached to it. After a simultaneous incubation at 4°C the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405 nm. The intensity of the bound yellow color is inversely proportional to the concentration of Oxytocin in either standards or samples. The measured optical density is used to calculate the concentration of Oxytocin. For further explanation of the principles and practice of immunoassays please see the excellent books by Chard or Tijssen. 2 Introduction Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology , more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-Vasopressin, maintain renal water and sodium balance. Oxytocin H-Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-NH2 Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and/or 8. In the oxytocin-like peptide, mesotocin, a common peptide found in some fishes, reptiles, amphibians, marsupials and nonmammalian tetrapods, the leucine at residue 8 is substituted for isoleucine8. Acting in classical endocrine fashion, Oxytocin elicits regulatory effects by binding specific cell surface receptors which in turn initiate a secondary intracellular response cascade via a phosphoinositide signaling pathway. 上海玉博生物技術(shù)有限公司在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國內(nèi)**地位，公司提供的產(chǎn)品涵蓋了二十多個(gè)國家近五十萬種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長和更新，公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對生物化學(xué)，分子生物學(xué)，細(xì)胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認(rèn)知，以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。
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