產(chǎn)品名稱：5a-Dihydrotestosterone (DHT) ELISA 產(chǎn) 地：Demeditec 產(chǎn)品貨號：DE2330 產(chǎn)品規(guī)格：96 Tests 產(chǎn)品說明： Special remarks: 5α-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a class called androgens. DHT is a C19 steroid and possesses androgenic activity. The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroids circulate in the unbound form in the blood and are referred to as the free fractions. DHT has at least three times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione. The major organ to neutralize androgens is the liver. Therefore in the liver the steroid hormones undergo structural modifications that are generally regarded as prerequisites for their biological inactivation. Some metabolites are formed and some are returned to the circulation before renal excretion. Therefore, elimination of steroids from the body is done through the urine. Clinical Trends: In Klinefelter's syndrome the DHT level is much lower than that found in normal men. In idiopathic hirsutism about 40% of the patients have an increased level of DHT. In polycystic ovaries (PCO) about 35% of the patients have an increased DHT level. The DHT level in young people is much higher than those found in normal older people, hence androgen production increases at puberty which gives rise to masculinizing characteristics. It has been demonstrated that the human testes produce DHT, which appears to originate in the seminiferous tubules. Therefore in tubular damage the production of DHT is impaired, which causes a decrease in the levels of plasma DHT (patients with germinal cell aplasia and azoospermia). There is a very low level of plasma DHT in patients with anorchia. It has been reported that in some prostate cancer (especially in stage D) the determination of DHT could be useful in predicting the response to anti-androgen therapy. This testkit coded DE2330 also can be used for veterinary investigations if the animal sample will be extracted using the following procedure: Extraction procedure for steroids We are recommending an extraction procedure as follows. 1.Pipette 1 ml of Ethyl Acetate in a 2.5 ml PP microtube (e.g. from Sarstedt). 2.Add between 5 and 200 µl of your sample. 3.Mix for 2-3 minutes on a vortexer. 4.Centrifuge the microtube briefly in order to achieve a clear separation of the 2 phases. 5.Deep freeze the microtube at -20°C or lower for several hours (or overnight). 6.Decant the organic liquid phase into a new tube which can be used for an evaporation process (the specification of the new tube depends of the specifications of your evaporating device). Be sure that no frozen material will be decanted. 7.Evaporate the organic solvent in an appropriate device at elevated temperature. The boiling point of Ethyl Acetate is 77°C. Please stay clearly below this temperature while evaporating. 8.After evaporating the organic solvent until complete dryness re-dissolve your sample with a diluent. In most cases this will be the zero standard of the ELISA (or RIA) to be used for the quantification of the analyte. 9.The volume of the diluent to be used for the reconstitution depends of the sensitivity of your assay system in relation to the analyte concentration in the primary sample. 10.The dilution (or concentration) factor depends of the volumes you have used in #1 and #7. 上海玉博生物技術(shù)有限公司在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國內(nèi)**地位，公司提供的產(chǎn)品涵蓋了二十多個國家近五十萬種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長和更新，公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對生物化學(xué)，分子生物學(xué)，細(xì)胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認(rèn)知，以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。
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