產(chǎn)品名稱：Leptin Multi-Species RIA 產(chǎn) 地：Demeditec 產(chǎn)品貨號：DE1874 產(chǎn)品規(guī)格：250 Tests 產(chǎn)品說明： Special remarks: This kit is for research purposes only! INTENDED USE The Multi-Species Leptin Radioimmunoassay (RIA) Kit has been developed to quantitate leptin in plasma or serum from many animals. The antibody used in this kit was raised against Human Leptin but displays broad crossreactivity to leptin molecules of many, but not all, species. It is recommended that investigators determine the suitability of this assay for the analysis of leptin in the species of interest. PRINCIPLES OF PROCEDURE In radioimmunoassay, a fixed concentration of labeled tracer antigen is incubated with a constant dilution of antiserum such that the concentration of antigen binding sites on the antibody is limited, for example, only 50% of the total tracer concentration may be bound by antibody. If unlabeled antigen is added to this system, there is competition between labeled tracer and unlabeled antigen for the limited and constant number of binding sites on the antibody. Thus, the amount of tracer bound to antibody will decrease as the concentration of unlabeled antigen increases. This can be measured after separating antibody-bound from free tracer and counting one or the other, or both fractions. A standard curve is set up with increasing concentrations of standard unlabeled antigen and from this curve the amount of antigen in unknown samples can be calculated. Thus, the four basic necessities for a radioimmunoassay system are: a specific antiserum to the antigen to be measured, the availability of a radioactive labeled form of the antigen, a method whereby antibody-bound tracer can be separated from the unbound tracer, and finally, an instrument to count radioactivity. The Multi-Species Leptin assay utilizes 125I-labeled Human Leptin and a Multi-Species Leptin antiserum to determine the level of Leptin in serum, plasma or tissue culture media by the double antibody/PEG technique. 上海玉博生物技術(shù)有限公司在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國內(nèi)**地位，公司提供的產(chǎn)品涵蓋了二十多個國家近五十萬種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長和更新，公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對生物化學(xué)，分子生物學(xué)，細胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認知，以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。
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