產(chǎn)品名稱：Progesterone ELISA
產(chǎn)    地：Demeditec
產(chǎn)品貨號：DE1561
產(chǎn)品規(guī)格：96 Tests
產(chǎn)品說明：
Special remarks:  This product has been 510(k) cleared by the FDA.
Progesterone (pregn-4-ene-3, 20-dione) is a C21 steroid hormone containing a keto-group (at C-3) and a double bond between C-4 and C-5 (Δ4).
This steroid hormone is a female sex hormone which, in conjunction with estrogens, regulates the accessory organs during the menstrual cycle and it is particularly important in preparing the endometrium for the implantation of the blastocyte and in maintaining pregnancy.
In non-pregnant women progesterone is mainly secreted by the corpus luteum whereas in pregnancy the placenta becomes the major source.
Minor sources are the adrenal cortex for both sexes and the testes for males.
Progesterone circulates in blood mainly bound to Corticosteroid Binding Globulin (CBG), Sex Hormone Binding Globulin (SHBG) and Albumin.
Only 2-10% of the total concentration circulates as free hormone.
Blood progesterone concentrations vary widely according to the phases of menstrual cycle; they are lower than 1 ng/ml (3.2 nmol/L) in the follicular phase and around 10-20 ng/ml (32 -64 nmol/L) in the luteal phase.
The maximal levels are achieved 4-7 days after ovulation and remain elevated for 4-6 additional days prior to falling to the preovulatory levels 24 hours before the onset of menstruation.
Since the rise and fall of progesterone parallel the activity of ovarian follicle and corpus luteum, measurements of plasma progesterone are clinically used to confirm ovulation and normal function of the corpus luteum in non-pregnant women.
If ovulation does not occur the corpus luteum is not formed and no cyclical rise of progesterone in plasma is observed. Abnormal progesterone secretion has been implicated in premenstrual tension, irregular shedding of endometrium, dysmenorrhoea, and luteal insufficiency.
Progesterone concentration can vary not only from subject to subject but also in the same person from day to day or even from hour to hour. Consequently, in gynecological disorders or abnormal pregnancies serial measurements rather than single ones are recommended for a proper interpretation of results.
During pregnancy progesterone is widely produced by placenta, and plasma levels rise steadily achieving values as high as 200 ng/ml at term.
The above test kits measures the total (unconjugated) Progesterone in serum. There is also a method available for the measurement of free Progesterone in saliva. The metabolic activity of any steroid is just represented by the free hormone fraction which represents less than 5% of the total. The non-invasive sampling of saliva samples offers another advantage of salivary testing. Demeditec is offering such a testkit in ELISA format with the catalogue number DES6633.
This testkit coded DE1561 also can be used for veterinary investigations if the animal sample will be extracted using the following procedure:
Extraction procedure for steroids
We are recommending an extraction procedure as follows.
1.Pipette 1 ml of Ethyl Acetate in a 2.5 ml PP microtube (e.g. from Sarstedt).
2.Add between 5 and 200 µl of your sample.
3.Mix for 2-3 minutes on a vortexer.
4.Centrifuge the microtube briefly in order to achieve a clear separation of the 2 phases.
5.Deep freeze the microtube at -20°C or lower for several hours (or overnight).
6.Decant the organic liquid phase into a new tube which can be used for an evaporation process (the specification of the new tube depends of the specifications of your evaporating device). Be sure that no frozen material will be decanted.
7.Evaporate the organic solvent in an appropriate device at elevated temperature. The boiling point of Ethyl Acetate is 77°C. Please stay clearly below this temperature while evaporating.
8.After evaporating the organic solvent until complete dryness re-dissolve your sample with a diluent. In most cases this will be the zero standard of the ELISA (or RIA) to be used for the quantification of the analyte.
9.The volume of the diluent to be used for the reconstitution depends of the sensitivity of your assay system in relation to the analyte concentration in the primary sample.
10.The dilution (or concentration) factor depends of the volumes you have used in #1 and #7.
上海玉博生物技術(shù)有限公司在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于國內(nèi)**地位，公司提供的產(chǎn)品涵蓋了二十多個國家近五十萬種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長和更新，公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對生物化學(xué)，分子生物學(xué)，細(xì)胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認(rèn)知，以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。

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