產品名稱：Testosterone ELISA 產 地：Demeditec 產品貨號：DE1559 產品規(guī)格：96 Tests 產品說明： Special remarks: This product has been 510(k) cleared by the FDA and therefore can be used for clinical purpose also in the US. Testosterone (17β-hydroxy-4-androstene-3-one) is a C19 steroid with an unsaturated bond between C?4 and C?5, a ketone group in C?3 and a hydroxyl group in the β position at C?17. This steroid hormone has a molecular weight of 288.47. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands. Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter's syndrome, Testicular feminization, Orchidectomy and Cryptorrchidism, enzymatic defects and some autoimmune diseases. The above test kits measures the total (unconjugated) Testosterone in serum. There is also a method available for the measurement of free Testosterone in saliva. The metabolic activity of any steroid is just represented by the free hormone fraction which represents less than 5% of the total. The non-invasive sampling of saliva samples offers another advantage of salivary testing. Demeditec is offering such a testkit in ELISA format with the catalogue number DES6622. This testkit coded DE1559 also can be used for veterinary investigations if the animal sample will be extracted using the following procedure: Extraction procedure for steroids We are recommending an extraction procedure as follows. 1.Pipette 1 ml of Ethyl Acetate in a 2.5 ml PP microtube (e.g. from Sarstedt). 2.Add between 5 and 200 µl of your sample. 3.Mix for 2-3 minutes on a vortexer. 4.Centrifuge the microtube briefly in order to achieve a clear separation of the 2 phases. 5.Deep freeze the microtube at -20°C or lower for several hours (or overnight). 6.Decant the organic liquid phase into a new tube which can be used for an evaporation process (the specification of the new tube depends of the specifications of your evaporating device). Be sure that no frozen material will be decanted. 7.Evaporate the organic solvent in an appropriate device at elevated temperature. The boiling point of Ethyl Acetate is 77°C. Please stay clearly below this temperature while evaporating. 8.After evaporating the organic solvent until complete dryness re-dissolve your sample with a diluent. In most cases this will be the zero standard of the ELISA (or RIA) to be used for the quantification of the analyte. 9.The volume of the diluent to be used for the reconstitution depends of the sensitivity of your assay system in relation to the analyte concentration in the primary sample. 10.The dilution (or concentration) factor depends of the volumes you have used in #1 and #7. 上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于國內**地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數(shù)量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫(yī)學領域的應用。
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