產(chǎn)品名稱：Mouse IL-17A/F ELISA (2 plates) 產(chǎn)品貨號：eBioscience 88-7272-22 產(chǎn)品規(guī)格：2 x 96 tests Mouse IL-17AF (heterodimer) ELISA Ready-SET-Go!® Also known as: Interleukin 17A RUO: For Research Use Only. Not for use in diagnostic procedures. SKU# 88-7272 Cat. No. Size 88-7272-22 2 x 96 tests w/plates 88-7272-88 10 x 96 tests 88-7272-86 10 x 96 tests w/plates Description: This mouse IL-17AF ELISA Ready-SET-Go! Reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse IL-17AF protein levels from samples including serum, plasma and supernatants from cell cultures. The assay demonstrates parallelism in measuring recombinant and native mouse IL-17AF proteins and a standard curve range of 30 to 4000 pg/ml. Minimal crossreactivity to the Il-17F homodimer is observed (<1%) and <7% crossreactivity to IL-17A homodimer when spiked in excess (100ng/ml). IL-17A and IL-17F are members of the IL-17 family and signature Th17 cytokines. Of the six IL-17 family members, IL-17F and IL-17A share the strongest homology (50% amino acid identity) and the two genes are located in the same chromosomal region. Although both IL-17A and IL-17F were originally found to be produced as disulfide-linked homodimers, recent studies have confirmed the production of IL-17A/IL-17F heterodimers in in vitro-differentiated and polarized Th17 cells. Activated human CD4+ T cells in culture were found to secrete IL-17F homodimer at 10-fold higher levels than IL-17A homodimer, suggesting that the majority of the IL-17A protein expressed exists in the form of the IL-17AF heterodimer. Studies of Th17 polarized mouse splenocytes also indicate the majority of IL-17 made is the IL-17AF heterodimer. IL-17F and IL-17A have been observed in tissue samples from various autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma.
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