產(chǎn)品名稱：Mouse IL-23 Ready-SET-Go!® (2nd Generation) 2 plates (includes plates) 產(chǎn)品貨號(hào)：eBioscience 88-7230-22 產(chǎn)品規(guī)格：2 x 96 tests w/plates Mouse IL-23 ELISA Ready-SET-Go!® (Second generation assay) Also known as: Interleukin-23, IL23, p40, p19 RUO: For Research Use Only. Not for use in diagnostic procedures. SKU# 88-7230 Cat. No. Size 88-7230-22 2 x 96 tests w/plates 88-7230-86 10 x 96 tests w/plates 88-7230-88 10 x 96 tests Description: This Mouse IL-23 (p19/p40) ELISA Ready-SET-Go! 'IS' ELISA Set (Improved sensitivity assay with 5B2 capture antibody) with high affinity binding ELISA plates contains the necessary reagents, buffers and diluents for performing quantitative, enzyme-linked immunosorbent assays (ELISA). The 5B2 antibody in combination with the C17.8 antibody yields an improved IL-23 sandwich ELISA with greater sensitivity than was achievable with previous assays. This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse IL-23 protein levels from samples including serum, plasma, and supernatants from cell cultures. The assay demonstrates parallelism in measuring recombinant and native mouse IL-23 proteins with a standard curve range of 4 pg/ml to 500 pg/ml and assay sensitivity below 4 pg/ml. Native mouse IL-23 was detected in supernatants from bone marrow-derived, LPS-activated dendritic cells. The use of a p19-specific capture antibody and a p40-specific detection antibody renders this assay exquisitely specific for mouse IL-23. IL-12 p40 homodimer and IL-12 p70 were each run in the assay at 500 ng/ml with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was run in the IL-23 ELISA at 100 ng/ml with no cross reactivity observed; all values were at the limit of detection of the assay. For measurement of total p40 protein levels, the Mouse IL-12/23 Total p40 ELISA Ready-SET-Go! is available (88-7120).
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