產品名稱：CREB (Total) InstantOne™ ELISA 產品貨號：eBioscience 85-86143-11 產品規(guī)格：96 tests beta-Catenin (Total/Phospho) InstantOne™ ELISA Also known as: CTNNB1 RUO: For Research Use Only. Not for use in diagnostic procedures. Attention: The Total/Phospho InstantOne ELISAs contain reagents for a total of 96 wells (24 wells total/72 wells phospho). Please contact Technical Service for a lot specific COA. SKU# 85-86143 Cat. No. Size 85-86143-11 96 tests Description: The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human, mouse, and rat beta-Catenin (Ser45) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate. beta-Catenin is a component of the WNT/Frizzled pathway. Beta-Catenin associates with the cytoplasmic domain of E-Cadherin, a protein that helps govern cell-cell adhesion, as well as the tumor suppressor adenomatous polypopsis coli (APC). GSK3 phosphorylates both beta-Catenin and APC, thereby marking them for degradation. Wnt binding to the Frizzled receptor induces GSK3 phosphorylation, which leads to inhibition of its kinase activity and subsequent reduction in ß-Catenin phosphorylation and degradation. As such, beta-Catenin translocates into the nucleus and functions as a co-activator of the TCF/LEF transcription factor family, which plays key roles in regulating genes involved in development and cancer progression.
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