產(chǎn)品名稱：anti-human Foxp3 Staining Set PE Set 236A/E7 Set 產(chǎn)品貨號：eBioscience 72-5774-40 產(chǎn)品規(guī)格：Kit Anti-Human Foxp3 Staining Set PE Also known as: Forkhead Box P3, Scurfin, JM2 Clone: 236A/E7 Set RUO: For Research Use Only. Not for use in diagnostic procedures. SKU# 72-5774 Cat. No. Size 72-5774-40 Kit Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to human Foxp3 include the recently published PCH101 (cat. 72-5776) and ebio7979 (cat. 13-7979). The 236A/E7 antibody reacts with human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3 Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population. The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). Not included: Flow Cytometry Staining Buffer (cat. 00-4222) Surface markers such as anti-CD4 (cat. 17-0049 or 11-0049) and APC anti-CD25 (cat. 17-0259) [For 72-5774 and 77-5774] Mouse IgG1 isotype control (cat. 12-4714 or 17-4714) [For 73-5774] Normal Mouse Serum (24-5544)
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