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產(chǎn)品名稱：anti-mouse/rat Foxp3 Staining Set FITC Set FJK-16s Set 產(chǎn)品貨號(hào)：eBioscience 71-5775-40 產(chǎn)品規(guī)格：Kit Anti-Mouse/Rat Foxp3 Staining Set FITC Also known as: Forkhead Box P3, Scurfin, JM2 Clone: FJK-16s RUO: For Research Use Only. Not for use in diagnostic procedures. SKU# 71-5775 Cat. No. Size 71-5775-40 Kit Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, and bovine Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human. Intracellular staining of mouse splenocytes with FJK-16s using the PE anti-mouse/rat Foxp3 Staining Set and protocol reveals approximately 2% of total cells in the C57Bl/6 strain and approximately 3-5% in the BALB/c mouse strain. Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+CD25+ cells and 4% of the CD4+CD25- cells staining with FJK-16s. B220+, CD11b+, CD11c+, and Ly6G/Gr-1+ cells do not show significant co-staining with FJK-16s. Please see our FAQ regarding the usage of eBioscience Foxp3 reagents. Not included: Fc Block (cat. 14-0161) Flow Cytometry Staining Buffer (cat. 00-4222) Rat IgG2a isotype Control (cat. 12-4321, 11-4321, or 17-4321)
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