產(chǎn)品名稱：Fura-2, AM 1 mg 產(chǎn)品貨號(hào)：eBioscience 65-0858-39 產(chǎn)品規(guī)格：1 mg Fura-2 AM Dye RUO: For Research Use Only. Not for use in diagnostic procedures. SKU# 65-0858 Cat. No. Size 65-0858-39 1 mg Description: Fura-2 is a calcium imaging dye that binds to free Ca2+. Fura-2 AM is the cell-permeable acetoxymethyl (AM) ester form of Fura-2. Fura-2 is the preferred dye for ratiometric imaging microscopy with digital image analysis, especially when the alteration of excitation wavelengths is more practical than the detection of multiple emission wavelengths. Upon binding Ca2+, the excitation spectrum of Fura-2 shifts to shorter wavelengths between 300 and 400 nm, while the peak emission remains steady around 510 nm. The Kd of Fura-2 is highly dependent on pH, temperature, ionic strength and viscosity of the cytosol, thus great care should be taken when non-standard conditions are used. Molecular weight: 1001.86 Peak excitation: variable depending on the concentration of free Ca2+, between 300 and 400 nm Peak emission: 510 nm Fura-2 AM should be reconstituted in high-quality, freshly opened DMSO. Once reconstituted, it should be protected from light and stored at -20°C. Avoid freeze-thawing.
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