產(chǎn)品名稱：Indo-1, AM 1 mg 產(chǎn)品貨號：eBioscience 65-0856-39 產(chǎn)品規(guī)格：1 mg Indo-1 AM Calcium Sensor Dye RUO: For Research Use Only. Not for use in diagnostic procedures. SKU# 65-0856 Cat. No. Size 65-0856-39 1 mg Description: Indo-1 AM Calcium Sensor Dye is a membrane-permeable dye used for determining changes in calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Once Indo-1 enters the cell, esterases cleave the AM group yielding a membrane-impermeable dye. Unbound Indo-1 has a peak emission at 485 nm. Upon binding calcium, the peak emission shifts down to 410 nm. In flow cytometry this can be measured over time and can be represented as a ratio of the two emission wavelengths. To optimize the ratio between the two emissions, unbound Indo-1 fluorescence should be collected using a filter above 485nm (525 nm is a good option) while bound Indo-1 fluorescence should be collected using a filter below 400 nm. Because the emission profile of Indo-1 is broad, multicolor flow analysis using eFluor ™ 450, eFluor™605NC or eFluor™ 650NC is not possible, however fluorochrome conjugated antibodies utilizing the 488nm or 633 nm are quite compatible with Indo-1. Molecular Weight: 1009.91 Peak Excitation: 346 nm Peak Emission: 475 nm Indo-1 AM should be reconstituted in high-quality, freshly opened DMSO. Recommended concentration: 1 mM. Once reconstituted, it should be protected from light and stored at -20°C; avoid freeze-thaws.
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