anti-mouse IL-17F Alexa Fluor® 647 eBio18F10 **
產(chǎn)品名稱：anti-mouse IL-17F Alexa Fluor® 647 eBio18F10 100 ug
產(chǎn)品貨號：eBioscience 51-7471-82
產(chǎn)品規(guī)格：100 ug
Anti-Mouse IL-17F Alexa Fluor® 647 (To Be Discontinued. Refer to Replacement Format eFluor® 660, cat. 50-7471)
Also known as: Interleukin-17F, IL17F
Clone: eBio18F10
RUO: For Research Use Only. Not for use in diagnostic procedures.
Attention: To Be Discontinued. Refer to Replacement Format eFluor® 660 Cat. No. 50-7471
SKU# 51-7471
Cat. No. Size
51-7471-80 25 ug
51-7471-82 100 ug
Description: The eBio18F10 antibody reacts with mouse IL-17F. IL-17F is a 37 kD homodimer of the IL-17 family and a signature Th17 marker. Of all the six IL-17 family members, IL-17F and IL-17A share the strongest homology (50% amino acid identity), and the two genes are located in the same chromosomal region. Recent studies have demonstrated coordinated regulation of IL-17A and IL-17F during Th17 differentiation. Expression of IL-17F and IL-17A has been detected in activated human peripheral blood lymphocytes, specifically by activated human CD4+ T cells. In addition to IL-17A, differentiated Th17 cells also produce IL-17F and IL-22 upon re-activation. Like IL-17A, IL-17F has been linked with inflammatory diseases. IL-17F and IL-17A expression has been observed in tissue samples from various autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma. IL-17F treatment of airway epithelium, vein endothelial cells, and fibroblasts has been reported to induce expression of IL-6, IL-8, GRO-α, ENA-78, TGF-β, MCP-1, G-CSF, GM-CSF, and ICAM-1.
Like IL-17A, IL-17F is a disulfide-linked homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found also in the TGF-β, BMP, and NGF superfamilies. The presence of the cysteine knot motif suggested the possibility of a heterodimeric structure, as was reported for TGF-β and inhibin/activin. Recent reports confirm that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found to produce the IL-17A/F heterodimer, along with the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-α secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100-fold lower than IL-17A). In the mouse, the IL-17A/F heterodimer (alone or in synergy with TNF-α) was found to regulate the expression of IL-6 and KC (mouse homolog of human GRO-α); this was found to be dependent on IL-17RA and TRAF6.
For intracellular staining and flow cytometric analysis with direct conjugates of anti-mouse IL-17F, use of the Foxp3 buffer system (cat. 00-5523) is recommended. Other buffers may yield varying results. For more information, please contact technical support at tech@ebioscience.com.
