產(chǎn)品名稱：COLUMBIA BLOOD AGAR BASE 哥倫比亞血瓊脂基礎 產(chǎn)品貨號：OXOID CM0331B 產(chǎn)品規(guī)格：500G Directions Add 39g to 1 litre of distilled water. Boil to dissolve the medium completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C ａnd add 5% sterile defibrinated blood. Storage conditions ａnd Shelf life Store the dehydrated medium at 10-30°C ａnd use before the expiry date on the label. Store the prepared plates of medium at 2-8°C. Technique Inoculate the culture suspected to be Clostridium perfringens in a straight line across a plate of Columbia sheep blood agar. Streak an overnight （or older） culture of Streptococcus agalactiae known to produce the CAMP factor at right angles to the first inoculation taking care that the lines do not touch. Incubate anaerobically at 35-37°C for 18-24 hours. A positive reverse CAMP test is indicated by the formation of an ``arrowhead’’ of haemolysis between the lines of the Clostridium perfringens ａnd Streptococcus agalactiae growth. Description Traditionally, blood agar bases have been either casein hydrolysate or meat infusion media. The advantage of the first lies in the rapid production of large colonies, ａnd of the second in clearly defined zones of haemolysis ａnd good colonial differentiation. Columbia Agar Base （CM0331） （ Ellner et al.1 ） combines the virtues of both these types of media to give an improved all-round performance. This new base has shown versatility ａnd superior performance in several applications. Brucella To prepare a selective medium add Brucella Selective Supplement SR0083 to 500ml of sterile, molten Columbia Blood Agar Base （CM0331）, containing 5-10% v/v inactivated horse serum ａnd 1% w/v dextrose2,3. Campylobacter ａnd Helicobacter To prepare a selective medium add: Campylobacter Selective Supplement （Skirrow） SR00694 or Campylobacter Selective Supplement （Butzler） SR00855,6 or Modified Butzler （ISO） Selective Supplement SR0214 or Campylobacter Selective Supplement （Blaser-Wang） SR00987,8,9 or Helicobacter pylori Supplement SR0147 to 500 ml of sterile, molten Columbia Agar Base （CM0331） containing Campylobacter Growth Supplement SR023210 ａnd 5-7% v/v horse or sheep blood （SR0048, SR0050 or SR0051）. Egg Yolk Emulsion Agar made using Oxoid Columbia Agar Base （CM0331） ａnd Egg Yolk Emulsion SR0047 has been shown to be a satisfactory isolation medium for Helicobacter pylori11.
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