33342 熒光染料 bisBenzimide H 33342
產(chǎn)品說(shuō)明：Hoechst 33342，也稱(chēng)bisBenzimide H 33342或HOE 33342。分子式為C27H28N6O·3HCl·3H2O，分子量為615.99，CAS Number 23491-52-3。 Hoechst 33342是一種可以穿透細(xì)胞膜的藍(lán)色熒光染料，對(duì)細(xì)胞的毒性較低。 Hoechst 33342染色常用于細(xì)胞凋亡檢測(cè)，染色后用熒光顯微鏡觀察或流式細(xì)胞儀檢測(cè)。Hoechst 33342也常用于普通的細(xì)胞核染色，或常規(guī)的DNA染色。 Hoechst 33342的*大激發(fā)波長(zhǎng)為346nm，*大發(fā)射波長(zhǎng)為460nm；Hoechst 33342和雙鏈DNA結(jié)合后，*大激發(fā)波長(zhǎng)為350nm，*大發(fā)射波長(zhǎng)為461nm。 溶解度： Hoechst 33342溶于水，溶解度可達(dá)20mg/ml。 用途：用于細(xì)胞核染色時(shí)，推薦的Hoechst 33342工作濃度為0.5-10μg/ml。
Sigma相關(guān)資料：
Sigma-Aldrich B2261 bisBenzimide H 33342 trihydrochloride
≥98% (HPLC and TLC)
Synonym:
bisBenzimide, HOE 33342, Hoechst 33342, 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride
CAS Number:
23491-52-3
Linear Formula:
C27H28N6O · 3HCl · xH2O
Molecular Weight:
561.93 (anhydrous basis)
Beilstein Registry Number:
1234011
EC Number:
245-691-1
MDL number:
MFCD00012678
PubChem Substance ID:
24891639
Description
Frequently Asked Questions
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Application
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry. Excitation max. = 346 nm Emission max. = 460 nm
Biochem/physiol Actions
Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
Properties
assay
solubility
H2O: 20 mg/mL
phosphate buffers: precipitates
suitability
passes application test for fluorescence
storage temp.
−20°C
Safety
Hazard Codes
Xn
Risk Statements
22-37/38
Safety Statements
36/37
WGK Germany
1
品名
產(chǎn)地
貨號(hào)
規(guī)格
單價(jià)
備注
HoechstNo. 33342 熒光染料
Sigma
B2261
25mg
詢價(jià)
現(xiàn)貨
滬公網(wǎng)安備 31011002002623號(hào)
