33258 熒光染料,Hoechst 33258,bisBenzimide H 33258,HOE 33258
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產(chǎn)品名稱: 33258 熒光染料,Hoechst 33258,bisBenzimide H 33258,HOE 33258
產(chǎn)品型號(hào): sigma B2883 5mg
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無(wú)相關(guān)文檔
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33258 熒光染料,Hoechst 33258,bisBenzimide H 33258,HOE 33258
33258 熒光染料,Hoechst 33258,bisBenzimide H 33258,HOE 33258
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33258 熒光染料,Hoechst 33258,bisBenzimide H 33258,HOE 33258
33258 熒光染料
產(chǎn)品說(shuō)明：
Hoechst 33258，也稱bisBenzimide H 33258或HOE 33258。分子式為C25H24N6O·3HCl，分子量為533.88，CAS Number 23491-45-4。
Hoechst 33258是一種可以穿透細(xì)胞膜的藍(lán)色熒光染料，對(duì)細(xì)胞的毒性較低。
Hoechst 33258染色常用于細(xì)胞凋亡檢測(cè)，染色后用熒光顯微鏡觀察或流式細(xì)胞儀檢測(cè)。Hoechst 33258也常用于普通的細(xì)胞核染色，或常規(guī)的DNA染色。
Hoechst 33258的*大激發(fā)波長(zhǎng)為346nm，*大發(fā)射波長(zhǎng)為460nm；Hoechst 33258和雙鏈DNA結(jié)合后，*大激發(fā)波長(zhǎng)為352nm，*大發(fā)射波長(zhǎng)為461nm。
溶解度：Hoechst 33258溶于水，溶解度可達(dá)10mg/ml。
用途：用于細(xì)胞核染色時(shí)，推薦的Hoechst 33258工作濃度為0.5-10μg/ml。
Sigma相關(guān)資料：
Sigma-Aldrich B2883 bisBenzimide H 33258
≥98% (HPLC and TLC)
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Synonym:
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BBIH, HOE 33258, Hoechst 33258, 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride
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CAS Number:
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23491-45-4
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Linear Formula:
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C25H24N6O · 3HCl
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Molecular Weight:
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533.88
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Beilstein Registry Number:
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4088183
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EC Number:
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245-690-6
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MDL number:
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MFCD00012679
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PubChem Substance ID:
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24891414
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Description
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Biochem/physiol Actions
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Membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.
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Application
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Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
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Properties
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assay
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≥98% (HPLC and TLC)
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solubility
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H2O: 10 mg/mL
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phosphate buffers: precipitates
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suitability
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passes application test for fluorescence
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storage temp.
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−20°C
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Safety
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Personal Protective Equipment
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dust mask type N95 (US), Eyeshields, Gloves
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Hazard Codes
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Xn
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Risk Statements
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22-36/38
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Safety Statements
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26-36
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WGK Germany
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3
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RTECS
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SM1140500
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品名
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產(chǎn)地
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貨號(hào)
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規(guī)格
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單價(jià)
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備注
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HoechstNo. 33258 熒光染料
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Sigma
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B2883
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25mg
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詢價(jià)
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現(xiàn)貨
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