Sigma R4875 Ribonuclease A,RNase A,Ribonuclease I,Pancreatic Ribonuclease, 核糖核酸酶I,核糖核酸酶A
Ribonuclease A 核糖核酸酶A
中文名：核糖核酸酶I,核糖核酸酶A
英文名：Ribonuclease,bovine pancrease,Ribonuclease I,Ribonuclease A,Pancreatic ribonuclease,Ribonucleate pyrimidinenucleotide-2′-transferase,Ribonucleate 3′-pyrimidino-oligonucleotidohydrolase,Rnase I,Rnase,Rnase A
分子量：相對(duì)分子質(zhì)量13000～15000
性狀：一種水解核糖核酸的酶。商品為白色結(jié)晶或凍干粉末,也有50%甘油的稠狀溶液。對(duì)濕敏感。極易溶于水。酶反應(yīng)：專一地催化核糖核酸的核糖部分3′和5′磷酸二酯鍵的裂開(kāi),形成具有2′,3′-環(huán)一磷酸衍生物寡聚核苷酸。水溶液在25℃以下相當(dāng)穩(wěn)定,*大穩(wěn)定范圍pH 2～4.5。*適pH 7.7。*適溫度65℃。等電點(diǎn)(pI)7.8。比旋光度 [α]25D-0.47°(以每mG氮計(jì),c=5,水中)。抑制劑有0.0005mol/L鎂(MG2+)、脫氧核糖核酸(競(jìng)爭(zhēng)性抑制劑)、變性脫氧核糖核酸(比天然的更有效)。
用途：生化研究,測(cè)定核酸的結(jié)構(gòu),脫氧核糖核酸和核糖核酸的降解。核糖核酸酶 A （Ribonuclease A）來(lái)源于牛胰，為高度專一性核酸內(nèi)切酶，可特異攻擊 RNA 上嘧啶核苷酸的C′3上的磷酸根和相鄰核苷酸的C′5之間的鍵，產(chǎn)物為3′嘧啶單核苷酸或以3′嘧啶核苷酸結(jié)尾的低聚核苷酸。可除去 DNA:RNA或RNA:RNA雜合體中未雜交的 RNA 區(qū);可用來(lái)確定 DNA 或 RNA 中單堿基突變的位置, 用標(biāo)記了的RNA探針與待檢含單堿基置換的DNA或RNA退火,RNA：RNA或RNA：DNA中的錯(cuò)配堿基可被RNaseA切割。廣泛用來(lái)去除 DNA 樣品中的 RNA 。
溶液配制：將RNase A溶解在0.001mol/l醋酸鈉(ph5.2)中,使終濃度為10mg/ml,加熱到100°C,15min.室溫下緩慢冷卻,用0.1體積的1M的Tris-Cl調(diào)整pH至7.4后,小管分裝保存在-20°C.
Sigma相關(guān)產(chǎn)品資料：
Sigma R4875 Ribonuclease A from bovine pancreas
Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein
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Synonym:
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Pancreatic Ribonuclease, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase, RNase A
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CAS Number:
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9001-99-4
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Enzyme Commission (EC) Number:
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3.1.27.5 ( BRENDA | IUBMB )
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EC Number:
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232-646-6
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MDL number:
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MFCD00132181
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Description
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Analysis Note
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Protein determined by E1%/280.
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Application
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• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples.
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Preparation Note
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Salt fractionated and chromatographically purified.
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性質(zhì)
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type
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Type I-A
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assay
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≥60% RNase A basis (SDS-PAGE)
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form
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powder
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mol wt
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mol wt ~13,700
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total impurities
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salt, essentially free
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foreign activity
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protease, essentially free
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storage temp.
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−20°C
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**性
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Personal Protective Equipment
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Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
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Safety Statements
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22-24/25
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WGK Germany
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3
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RTECS
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RF0760000
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品名
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產(chǎn)地
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貨號(hào)
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規(guī)格
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單價(jià)
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備注
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RNase A 核糖核酸酶A
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Sigma
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R4875
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25mg
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詢價(jià)
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現(xiàn)貨
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RNase A 核糖核酸酶A
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Sigma
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R4875
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50mg
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詢價(jià)
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現(xiàn)貨
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RNase A 核糖核酸酶A
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Sigma
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R4875
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100mg
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詢價(jià)
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現(xiàn)貨
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