Trypsin inhibitor,from soybean,Kunitz soybean trypsininhibitor, SBTI,胰朊酶抑制劑, 胰蛋
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產(chǎn)品名稱: Trypsin inhibitor,from soybean,Kunitz soybean trypsininhibitor, SBTI,胰朊酶抑制劑, 胰蛋
產(chǎn)品型號(hào): Sigma T9003 25mg
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
Trypsin inhibitor,from soybean,Kunitz soybean trypsininhibitor, SBTI,胰朊酶抑制劑, 胰蛋白酶抑制劑
Trypsin inhibitor,from soybean,Kunitz soybean trypsininhibitor, SBTI,胰朊酶抑制劑, 胰蛋
的詳細(xì)介紹
Trypsin inhibitor,from soybean,Kunitz soybean trypsininhibitor, SBTI,胰朊酶抑制劑, 胰蛋白酶抑制劑
胰蛋白酶抑制劑
中文名：胰朊酶抑制劑, 胰蛋白酶抑制劑
英文名：Trypsin inhibitor,from soybean,Kunitz soybean trypsininhibitor, SBTI
分子量：相對(duì)分子質(zhì)量約 20100
CAS：9035-81-8
性狀：無(wú)鹽冷凍干粉或粗制粉末。對(duì)濕敏感。可溶于水和稀緩沖液。酶反應(yīng)：胰蛋白酶抑制劑＋胰蛋白酶(過(guò)量)→[抑制劑·胰蛋白酶]＋胰蛋白酶(剩佘)、 苯甲酰精氨酸乙酯剩余胰蛋白酶苯甲酰精氨酸＋乙醇。除胰蛋白酶外，還能抑制凝血因子Xa、血纖維蛋白溶酶、血漿激肽釋放酶。不抑制金屬蛋白酶、巰基蛋白酶、酸性蛋白酶、組織激肽釋放酶。等電點(diǎn)pI 4.5。*大吸收波長(zhǎng) 280nm。能被強(qiáng)酸、強(qiáng)堿變性，在40℃以上可逆變性。在pH 1.0～12.0稀 的緩沖液中穩(wěn)定。
用途：用親和色譜法精制胰蛋白酶。血纖維蛋白/血纖維蛋白原無(wú) 降解鑒定試驗(yàn)。
Sigma相關(guān)產(chǎn)品信息
Sigma T9003 Trypsin inhibitor from Glycine max (soybean)
Type I-S, lyophilized powder
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Synonym:
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SBTI
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CAS Number:
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9035-81-8
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EC Number:
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232-906-9
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MDL number:
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MFCD00082103
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Description
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Analysis Note
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Protein determined by biuret.
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One mg will inhibit 1.0-3.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.
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Other Notes
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Extremely stable: a 1% sterile filtered solution maintained activity for over 3 years when kept refrigerated.
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View more information on Trypsin Inhibitor.
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Preparation Note
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Chromatographically prepared
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Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.
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Unit Definition
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One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
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One chymotrypsin unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
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Biochem/physiol Actions
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Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108.
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性質(zhì)
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type
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Type I-S
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form
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lyophilized powder
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mol wt
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α-chain mol wt 20090 Da by calculation
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β-chain mol wt 20036 Da by calculation
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γ chain mol wt 20163 Da by calculation
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EQP level
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Premium
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solubility
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H2O: 10 mg/mL
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phosphate buffer: 10 mg/mL (hazy above 10 mg/ml)
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storage temp.
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2-8°C
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**性
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Personal Protective Equipment
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Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
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WGK Germany
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3
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品名
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產(chǎn)地
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貨號(hào)
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規(guī)格
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單價(jià)
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備注
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Trypsin Inhibitor 胰蛋白酶抑制劑
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Sigma
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T9003
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25mg
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詢價(jià)
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現(xiàn)貨
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Trypsin Inhibitor 胰蛋白酶抑制劑
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Sigma
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T9003
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100mg
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詢價(jià)
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現(xiàn)貨
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