Horseradish Peroxidase, HRP,Peroxidase,Donor:hydrogen-peroxide oxidoreductase,
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產(chǎn)品名稱: Horseradish Peroxidase, HRP,Peroxidase,Donor:hydrogen-peroxide oxidoreductase,
產(chǎn)品型號: Sigma P6782 10mg
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Horseradish Peroxidase, HRP,Peroxidase,Donor:hydrogen-peroxide oxidoreductase, Horseradish 現(xiàn)貨
Horseradish Peroxidase, HRP,Peroxidase,Donor:hydrogen-peroxide oxidoreductase,
的詳細介紹
Horseradish Peroxidase, HRP,Peroxidase,Donor:hydrogen-peroxide oxidoreductase, Horseradish 現(xiàn)貨
辣根過氧化物酶
過氧化物酶，酶學(xué)分類號為EC 1.11.1.7。該酶催化
Donor+ H2O2--→Oxidized donor+2 H2O。
過氧化物酶，通常來源于辣根（因此稱辣根過氧化物酶），是臨床檢驗試劑中的常用酶。該產(chǎn)品不但廣泛用于多個生化檢測項目，也廣泛運用于**類（ELISA）試劑盒。過氧化物酶作為多個試劑盒顯色體系的關(guān)鍵成分，對試劑盒的質(zhì)量有重要影響。
辣根過氧化物酶(Horseradish Peroxidase, HRP)比活性高，穩(wěn)定，分子量小，純酶容易制備，所以*常用。HRP廣泛分布于植物界，辣根中含量高，它是由無色的酶蛋白和棕色的鐵卟啉結(jié)合而成的糖蛋白，糖含量18％。HRP由多個同功酶組成，分子量為40,000,等電點為PH3～9,酶催化的*適PH因供氫體不同而稍有差異，但多在PH5左右。酶溶于水和58％以下飽和度硫酸銨溶液。HRP的輔基和酶蛋白*大吸收光譜分別為403nm和275nm，一般以OD403nm ／OD275nm的比值RZ(德文Reinheit Zahl)表示酶的純度。高純度的酶RZ值應(yīng)在3.0左右(*高可達3.4)。RZ值越小，非酶蛋白就越多。
Sigma相關(guān)產(chǎn)品資料：
Sigma P6782 Peroxidase from horseradish
Type VI-A, essentially salt-free, lyophilized powder, ~1000 units/mg solid (using ABTS), 250-330 units/mg solid (using pyrogallol)
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Synonym:
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Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase
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CAS Number:
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9003-99-0
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Enzyme Commission (EC) Number:
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1.11.1.7 ( BRENDA | IUBMB )
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EC Number:
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232-668-6
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MDL number:
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MFCD00071339
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Description
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Analysis Note
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The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.
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This product is assayed using ABTS for easy comparison to other suppliers' unit: approx. 1,000 units per mg solid
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Linkage
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Similar to P8375
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Other Notes
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View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.
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Packaging
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Packaged in mg solid
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Unit Definition
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One ABTS unit will oxidize 1 μmole of ABTS per minute at 25 °C at pH 5.0
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One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
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General description
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Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.
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性質(zhì)
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type
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Type VI-A
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form
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essentially salt-free, lyophilized powder
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EQP level
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Premium
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storage temp.
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2-8°C
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**性
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Personal Protective Equipment
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Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
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WGK Germany
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3
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品名
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產(chǎn)地
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貨號
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規(guī)格
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單價
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備注
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HRP辣根過氧化物酶
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Sigma
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P6782
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10mg
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詢價
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現(xiàn)貨
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HRP辣根過氧化物酶
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Sigma
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P6782
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25mg
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詢價
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現(xiàn)貨
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HRP辣根過氧化物酶
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Sigma
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P6782
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50mg
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詢價
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現(xiàn)貨
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HRP辣根過氧化物酶
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Sigma
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P6782
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100mg
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詢價
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現(xiàn)貨
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