地高辛標(biāo)記檢測(cè)試劑盒II,DIG-High Prime DNA Labeling and Detection Starter Kit II
地高辛標(biāo)記檢測(cè)試劑盒II
比普通DIG隨機(jī)引物法標(biāo)記效率高, 操作簡單, 可用于Southern、Northern和斑點(diǎn)雜交以及菌落和噬菌體篩選。一個(gè)Kit可用于12次標(biāo)記反應(yīng)和24次檢測(cè)反應(yīng)。-20℃保存。
· 隨機(jī)引物法標(biāo)記探針
· AP標(biāo)記抗體
· 底物NBT/BCIP
· 化學(xué)發(fā)光法檢測(cè)
Roche相關(guān)資料:
產(chǎn)品英文名稱:DIG-High Prime DNA Labeling and Detection Starter Kit II
Cat. No. : 11585614910
Pack size: 1 kit for 12 labeling reactions and detection of 24 blots 10 ng to 3 μg per assay, blots of 100 cm2
Convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Application
DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.
Product Description
Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
Contents
- DIG-High Prime, 5x conc., 50 µl
- DIG-labeled Control DNA, 20 µl, (5 µg/ml) pBR328 (linearized with Bam HI)
- DNA Dilution Buffer, 3 x 1 ml
- Anti-digoxigenin-AP Conjugate, 50 µl
- CSPD, ready-to-use, 50 ml
- Blocking Solution, 10x conc., 4 x 100 ml
- DIG Easy Hyb Granules, 4 x 100 ml
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DIG-High Prime DNA Labeling and Detection Starter Kit II
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Roche
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11585614910
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1 kit (12 labeling reactions and 24 detection reactions)
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4998
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現(xiàn)貨
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